![]() ![]() Trinity partitions the sequence data into many individual de Bruijn graphs, each representing the transcriptional complexity at a given gene or locus, and then processes each graph independently to extract full-length splicing isoforms and to tease apart transcripts derived from paralogous genes. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied sequentially to process large volumes of RNA-seq reads. Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Use the documentation links in the right-sidebar to navigate this documentation, and contact our Google group for technical support. Trinity -seqType fq -left reads_1.fq -right reads_2.fq -CPU 6 -max_memory 20Gįind assembled transcripts as: 'trinity_out_dir/Trinity.fasta' How can I run this in parallel on a computing grid?.How do I identify the specific reads that were incorporated into the transcript assemblies?.How do I use reads I downloaded from SRA.There are too many transcripts! What do I do?. ![]()
0 Comments
Leave a Reply. |